Identificación de fitocompuestos antioxidantes en el extracto acuoso de Phyllanthus orbicularis Kunth
Palabras clave:
P. orbicularis, 2,4-di-ter-butilfenol, OO’-difenol-4,4’,6,6’-tetra-ter-butil, 2,6-di-sec-butilfenol, anti- mutagénesis, daño oxidativo, fraccionamiento químicoResumen
The aqueous extract of Phyllanthus orbicularis contains bioproducts able to
significantly reduce the mutagenesis mediated by oxidative compounds. A
bioactive-directed fractionation of the aqueous extract of P. orbicularis has
been applied with the aim of identifying antimutagenic fractions, which al-
lowed isolating and identifying some biocompound responsible for this activ-
ity. Antimutagenesis of all fractions was determined by the Salmonella
retromutation assay, using hydrogen peroxide as model mutagen. Applying
this methodology, two potent antimutagenic fractions have been obtained
able to totally abolish the mutagenicity produced by hydrogen peroxide. In
these fractions 2,4-di-tertbutylphenol, OO’-diphenol-4,4’,6,6’-tetra-tert-butyl
and 2,6-di-sec-butylphenol were identified by Gas Chromatography/Mass
Spectrometry
