STATISTICAL STUDY OF THE FACTORS OF RISK AND DETERMINATION OF PSEUDOMONAS AERUGINOSA BY CONVENTIONAL PCR IN BEEF MARKETED IN THE HUÁNUCO MARKETS, PERU

Abstract

This paper aims to identify risk factors and to determine the level of contamination of Pseudomonas aeruginosa by conventional
Polymerase Chain Reaction (PCR) in beef commercialized in the markets of Huánuco, Peru. To meet this objective, an
epidemiological study was carried out. The sample consisted of 300 biological analyses of beef meats in the markets of
Huánuco. A sample was taken from each position, making a total of 300 during a few days. Observation guides were used in
order to collect data. The determination of the presence of Pseudomonas aeruginosa was made by PCR. A statistical analysis
was carried out using contingency tables and Chi-square Pearson's test, between the possible causes of contamination and the
results obtained from the sample analysis. Pseudomonas aeruginosa was isolated in 69 samples out of a total of 300,
corresponding to a frequency of 23%. Among the identified factors are: dirty uniform of another color (p=2.5469x10-13); dirty
hands (p=0.000); work utensils in poor condition and dirty (p=3.4776x10-12); these factors were statistically significant to the
frequency of Pseudomonas aeruginosa in beef.